Myelin Basic Protein重组抗体[IGX3421]_Myelin Basic Protein...

来自 : www.abcam.cn/myelin-basic-prot 发布时间：2021-03-25

All lanes : Anti-Myelin Basic Protein antibody [IGX3421] (ab209328) at 0.25 µg/ml

Lane 1 : Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2 : Mouse brain tissue lysate at 10 µg
Lane 3 : Rat brain tissue lysate at 10 µg
Lane 4 : Myelin Basic Protein (Recombinant protein) at 0.1 µg

Secondary
All lanes : HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 33 kDa
Observed band size: 18,23,24 kDa why is the actual band size different from the predicted?

Exposure time :

Lane 1 : 30 seconds.

Lanes 2-3: 2 minutes.

Lane 4: 8 minutes.

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209328 overnight at 4 C. Antibody binding was visualised using ECL development solution ab133406.

Human monoclonal [IGX3421] to Myelin Basic Protein Suitable for: ELISA, WB...The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the...购买重组Myelin Basic蛋白人单克隆抗体[IGX3421](ab209328)，Myelin Basic蛋白抗体经WB验证，可与人,小鼠,大鼠样本反应。3篇文献引用，5个独立用户反馈。Myelin Basic Protein重组抗体[IGX3421]